Insights

What Small Biotechs Get Wrong About First‑in‑Human Trials

Written by Sara Armani | Aug 11, 2026, 2:08:46 AM

For many small biotechs, the journey to firstinhuman (FIH) studies is both exhilarating and overwhelming. Its the moment in which years of discovery work finally meet clinical reality and where investors expect to see real, defensible progress. But FIH trials have changed dramatically over the past decade. What was once a relatively simple SAD/MAD progression has evolved into a far more complex, multipart exercise that demands strategic planning, regulatory awareness, and operational sophistication.

Yet many earlystage companies still approach FIH as if it were the same straightforward milestone it used to be. The result is predictable: delays, redesigns, regulatory pushback, and unnecessary cost.

Before any protocol is drafted, sponsors need to answer a set of foundational questions: Do the nonclinical data genuinely support entry into humans? How should the starting dose be justified NOAEL, MABEL, or a hybrid approach? How many cohorts will be required to characterise safety and pharmacology? And critically, which population makes sense for first dosing healthy volunteers, patients, or a combination and what are the risks and benefits of each path? These early decisions shape everything that follows.

Working across hundreds of earlyphase programmes, the hVIVO/CRS team sees the same misconceptions again and again. Here are the most common pitfalls and how small biotechs can avoid them.

1. “A first‑in‑human trial is simple - just a SAD and a MAD, right?”

Not anymore.

Twenty years ago, a typical FIH study involved a singleascendingdose (SAD) phase followed by a multipleascendingdose (MAD) phase. Today, investors, and development teams expect far more integrated designs. Modern FIH protocols often combine:

  • SAD
  • MAD
  • foodeffect assessments
  • patient cohorts
  • parallel arms
  • biomarker sampling, and
  • early PD readouts.

This shift reflects a broader industry trend: compressing timelines by gathering far more information earlier in development. But it also means FIH trials are no longer “plugandplay.” They demand thoughtful sequencing, strong safety oversight, and the kind of operational flexibility that only experienced earlyphase sites can provide. Many small biotechs underestimate just how much the landscape has evolved — and overestimate how ready they are to manage that level of complexity.

2. “We already know what our FIH design should look like.”

Sponsors frequently arrive with a draft synopsis in hand. Sometimes it’s close. More often, it needs significant refinement.

Common gaps include:

  • unclear doseescalation logic
  • not following GMP rules for the IMP production
  • unrealistic cohort sizes
  • missing safety stopping rules
  • inappropriate inclusion/exclusion criteria
  • misunderstanding of what EU or UK regulators expect
  • miscalculating the amount of study medicines needed
  • incorrect assumptions about healthy volunteer vs patient cohorts, and
  • timelines that don’t reflect operational reality.

This isn’t a failure — it’s simply the reality of earlystage biotech. Most small teams dont have inhouse clinical pharmacologists, medical directors, or regulatory strategists. The most successful biotechs are the ones who seek expert input early — not the ones who assume they already have the answers.

3. “Regulators will accept whatever we submit.”

Regional nuance matters — a lot.

USbased sponsors, in particular, are often surprised by how differently EU and UK authorities evaluate FIH protocols. German regulators, for example, have specific expectations around safety monitoring, doseescalation justification, stopping criteria, risk mitigation, patient inclusion in early cohorts, and justification of both the starting and highest planned dose.

A synopsis that looks perfectly reasonable in Boston may raise immediate questions in Berlin. hVIVO/CRS teams routinely help sponsors reshape synopses to align with local expectations — often preventing weeks or months of regulatory delay.

For novel or higherrisk products, or when nonclinical data leave areas of uncertainty, early engagement with regulators becomes even more important. Planning and participating in prescientific meetings can clarify expectations, derisk key decisions, and ensure that the proposed FIH design is acceptable before a full submission is made.

These regional differences often surprise firsttime sponsors, and they can shape the entire trajectory of an earlyphase programme. FIH success depends not only on strong science, but on knowing exactly what each authority expects to see and addressing uncertainties proactively.

4. “We’ll figure out the details once we’re closer to the trial.”

This is one of the costliest misconceptions.

Small biotechs often approach CRS two years before their planned FIH study — not because they’re ready to start, but because they need:

  • feasibility insights
  • preliminary costings
  • realistic timelines
  • early design guidance, and
  • input for investor decks.

Early engagement is invaluable because it prevents teams from drifting into designs that aren’t feasible, making promises to investors they can’t keep, underestimating how long key steps will take, or discovering too late that their protocol needs major amendments.

FIH planning should begin long before the IND/CTA submission — not after.

5. “We don’t need help - we’ve got this.”

This misconception is especially common among small biotechs with strong scientific founders. They know their molecule better than anyone — but FIH trials require a different kind of expertise.

FIH success depends on:

  • clinical judgement
  • operational nuance
  • regulatory familiarity
  • riskbased decisionmaking
  • realworld feasibility

These are not skills most earlystage teams have inhouse nor should they be expected to. The strongest biotechs are the ones who recognise where they need support and build the right partnerships early, especially in the firstinhuman stage.

6. “Any Phase I unit can run our study.”

FIH trials are not interchangeable across sites. Modern FIH studies require:

  • experienced clinical pharmacologists
  • physicians trained in earlyphase risk management
  • hospital-based facilities proximate to an intensive care unit
  • teams comfortable with complex, multipart designs
  • seamless coordination between medical, operational, and regulatory functions

hVIVO’s earlyphase units are built for exactly this kind of complexity. Our earlyphase specialist ecosystem brings together the disciplines that matter most at the FIH stage clinical pharmacology, regulatorysavvy protocol design, biostatistics, specialised patient access, laboratory science, and tightly coordinated clinical operations and integrates them into a single, connected workflow. Instead of handing a study off between disconnected vendors and fragmented teams, every part of the programme moves through a team that works together every day, shares data seamlessly, and understands how earlyphase decisions shape laterphase success.

That’s the advantage of a specialist ecosystem: fewer gaps, fewer surprises, and a smoother, more reliable path through firstinhuman than a generalist CRO can offer.

The Bottom Line

Firstinhuman trials are no longer simple milestones. They are complex, highstakes clinical investigations that demand strategic planning, regulatory insight, and operational excellence.

Small biotechs that recognise this — and who engage early with experienced earlyphase teams move faster, reduce risk, and generate cleaner, more decisionready data. Those who dont often learn the hard way.

And this is where an earlyphase specialist ecosystem becomes a genuine advantage. When clinical pharmacology, regulatory expertise, biostatistics, laboratory science, and operational delivery work as one coordinated unit, the entire programme moves with greater confidence and fewer surprises giving emerging biotechs the support they need to navigate firstinhuman successfully.